WebIHC staining of mouse brain using 15012-1-AP. Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15012-1-AP (MYO1B Antibody) at dilution of 1:50 (under 40x lens). Save time and replace your secondary antibodies with our FlexAble Antibody Labeling Kits. WebThe MYO5B gene provides instructions for making a protein called myosin Vb. This protein is one of a group of proteins with similar structures called myosins, which are involved in …

Aberrant Myosin 1b Expression Promotes Cell …

WebMay 26, 2024 · MYO1B interacts either directly or indirectly with the transmembrane tyrosine kinase receptors EPHB2, which bind ephrins to regulate processes such as cell repulsion, migration, proliferation, and cell-cell adhesion during embryogenesis (Prosperi et al. 2015; Kania and Klein 2016 ). We measured the protein surface density (number of proteins per unit area) on solid surfaces or on SLBs by using a previously established procedure36,37. It is calculated from a labeled proteins/lipids calibration. We first measure the fluorescence of POPC SLBs containing predefined amounts of Atto488-DOPE … See more Actin was purified from rabbit muscle and isolated in monomeric form in G buffer (5 mM Tris-HCl, pH 7.8, 0.1 mM CaCl2, 0.2 mM ATP, 1 mM DTT and 0.01% NaN3). Actin was labeled with Alexa 594 succimidyl ester … See more SLBs were formed by fusion of small unilamellar vesicles (SUVs) prepared as follows. Lipid mixtures containing 79.5% POPC, 20% L-α-phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) and 0.5% Rhodamine-PE or … See more The kinetics of single filament assembly was monitored by TIRF microscopy (Eclipse Ti inverted microscope, 100× TIRF objectives, Quantem … See more Lipid compositions for GUVs were 79.7% POPC, 20% L-α-phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) and 0.3% Texas Red DHPE. GUVs were prepared by using polyvinyl alcohol … See more crumstane farmhouse

Myosin 1b promotes cell proliferation, migration, and

WebSep 27, 2024 · MYO1B Prevents PTEN Nuclear Localization by Binding to PTEN Previous study indicates that MYO1B might also act as a dynamic scaffold in interacting directly or indirectly with other proteins to modulate the trafficking of … Webextracellular exosome. cell periphery. myosin complex. plasma membrane. apical part of cell. Biological process. actin filament organization. actin filament-based movement. … WebIn vitro, knockdown of Myo1b significantly suppressed proliferation, migration, and invasion of CaSki and SiHa cells, and markedly decreased the MMP1/MMP9 activities. … build your own stainless steel cookware