Easytaq buffer

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August undefined, 2024

http://www.life-tech.org/2xeasytaq-pcr-supermix/ WebEasyTaq® DNA Polymerase is isolated from E. coli expressing a cloned DNA polymerase gene from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. ... Storage Buffer. 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. Storage-20°C for two years ...

2x EasyTaq PCR SuperMix (+dye) - AS111 Civic Bioscience

WebNov 16, 2024 · We performed the PCR experiment using 20 μl reaction mixtures containing 100 ng genomic DNA, 2 μl 10× EasyTaq Buffer (with Mg 2+), 1.5 μl 2.5 mM dNTPs, 2 μl each of 2 μM primer stock, and 1 U EasyTaq DNA Polymerase (TransGen Biotech, Beijing, China) under the following thermal cycler conditions: 95°C for 5 min, then 35 cycles at … quite good writing editing

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WebJun 22, 2024 · to the EasyTaq DNA polymerase. However, poten- ... Among the four lysis buffers examined, Y-PER is observed to be more effective than Tris/EDTA, 0.2 % SDS, and 10 mM EDTA in the extraction of PCR ... Web10×EasyTaq® Buffer 5 μl 1× 2.5 mM dNTPs 4 μl 0.2 mM EasyTaq® DNA Polymerase 0.5-1 μl 2.5-5 units Nuclease-free Water Variable - Total volume 50 μl - Reaction … WebOct 26, 2024 · A rapid, convenient, low-cost, and selective DNA isolation method was developed for identifying meat adulteration. A mesoporous metal organic framework (Meso-UIO-66)-coated solid phase microextraction system was employed as an isolation device to simplify DNA isolation into three steps (lysis, washing, and elution). Meso-UIO-66 was … shire of ravensthorpe fees \\u0026 charges

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WebThe 10x EasyTaq buffer is used with EasyTaq DNA Polymerase. Additional information. Supplier: Transgen Biotech. Format: 1.2 ml. Ask a question about 10x EasyTaq Buffer (with Mg2+) – GK101. Name. Email Address. Competitor product (if applicable) Question. Get an answer quickly. WebJun 23, 2024 · PCR system was composed of 2.5 μl of 10 x EasyTaq ® Buffer, 2 μl of 2.5 mM dNTPs, 0.5 μl of EasyTaq DNA polymerase, 0.5 μl of 10 μm each primer, genomic DNA of each species, and refilled ddH 2 O to 25 μl in a single reaction. The reaction was elicited by a 5-min denaturation at 94°C, followed by 34 cycles (94°C for 30 s, 63°C for 30 s ... quite good foodWebApr 4, 2016 · The PCR amplification was performed in 20 µL of reaction mixture: 1 µL genomic DNA, 0.5 µL of each of the 4 primers (10 µmol/L), 2 µL of dNTPs (2.5 mmol/L), 2 µL of 10× EasyTaq buffer (Mg 2+ plus), 1 µL of EasyTaq polymerase (5 U/µL, TransGen), and 12 µL of ddH 2 O. The thermal profile was the following: an initial denaturation step ... quite impartially crossword clue

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Easytaq buffer

WebSep 19, 2024 · To determine the optimal pH for the acetophenone asymmetric bioreduction system using M190V, a range of buffers was used, with pH varying from 5.5 to 10.5. As illustrated in Figure 4c, when the buffer pH increased from 5.5 to 6, the conversion of acetophenone was obviously improved, and relatively high conversions (24.38–34.79%) … WebSep 16, 2024 · PCR reaction system (25 μL) included 2.5 μL of 10 × EasyTaq ® Buffer, 2 μL of 2.5 mM dNTPs, 0.5 μL of 5 U/μL EasyTaq DNA Polymerase, 0.5 μL of 10 μM each primer pair of eight species, DNA mixture of eight species at the indicated concentration and refilled ddH 2 O to 25 μL. PCR amplification was performed by T100™ Thermal Cycler ...

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WebAug 19, 2024 · The 20 μl PCR reaction mixture contained 10× EasyTaq buffer (2 μL), 2.5 mM dNTPs (1.6 μl), 5 U/μl EasyTaq DNA Polymerase (0.2 μl), 20 μM primers (1 μL each), crude DNA template (1 μl), and sterile distilled water. Amplification was performed on a thermal cycler with a cycling profile of initial denaturation at 95°C for 5 min; 30 ... WebEasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous …

WebTaKaRa Taq DNA Polymerase is a recombinant-version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.. For … Web- EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase. - Extension rate is about 0.5 kb/min. ... DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4 Unit Definition One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable …

WebThe SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H 2 O. • TransTaq ® High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq ® DNA Polymerase. • Extension rate is about 1-2 kb/min. • Template-independent "A" can be generated at the 3' end of the ... WebEasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×. 2×EasyTaq® PCR …

WebEasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×.

WebEasyTaq buffer (20 mM TrisHCl, pH 8.0, 200 mM KCl, 100 mM (NH 4) 2 SO 4, 20 mM MgSO 4, 0.6 µL of each forward and reverse primer (10 µM), 0.35 µL of 5 U/µL EasyTaq DNA polymerase). PCR amplifica tion was performed on thermal cycler (BioRad S1000, United States) using with the following condi tions: initial denaturation for 5 min at 94°C ... shire of ravensthorpe contactWebSep 25, 2024 · PCR was carried out in 50 μL reactions, including the template DNA (10 ng), 5 μL of 10 × EasyTaq buffer, 2 μL of 2.5 mM dNTPs, 1 μL of EasyTaq DNA polymerase (5 units), 1 μL of each primer (10 μM), and nuclease-free water. The PCR conditions were as follows: 95 °C for 2 min, 30 cycles of 95 °C for 30 s, 55 °C for 30 s, and 72 °C for ... quite happy crosswordWebA 2 μl suspension was used as template in PCR reactions. PCR reactions were performed in a 20 μl volume containing 2 μl 10 × EasyTaq buffer, 200 μM dNTPs, 0.2 μM of each primer, and 1 U EasyTaq DNA polymerase (TransGen Biotech, Beijing, China). The primers used in this study were listed in Table 1. The PCR procedures were: 95 °C for 3 ... quite elderly meansWeb摘要：2024年5月，江苏扬州某鸭场25日龄左右樱桃谷鸭发生了以瘫痪为主要表现的神经症状疾病，病死鸭剖检无特征性病变。RT-PCR检测结果显示为鸭坦布苏病毒（T,现代畜牧cvonet,中国畜牧,特种养殖,肉食品,猪价,种猪,仔猪,生猪,西门塔尔,波尔山羊,鸡蛋,鸭蛋,饲料,玉米,兽药,养殖设备,孵化器,行情,标准 ... shire of ravensthorpe little barrensWebAug 31, 2024 · The reaction system was prepared using the EasyTaq® DNA Polymerase kit (TransGen Biotech, Beijing, China): 1 μl EasyTaq® DNA Polymerase, 5 μl template, 2 μl 10× EasyTaq® Buffer, 2 μl 2.5 mM dNTPs, 2 μl primer mix, 8 μl ddH 2 O. Each 100 μl of primer mix contains 1 μl of primers of all the 33 targets bacterium, IC, and human DNA ... quite frankly in text speechWebTaKaRa Taq DNA Polymerase is a recombinant-version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.. For individual reaction setup and optimization, use individual components of enzyme, dNTP mixture, and 10X reaction buffer (with or without Mg 2+).A convenient 2X PCR master mix of Takara … quite imposing plus 4 for mac downloadWeb10×EasyTaq® Buffer provides the preferred pH and ionic strength for PCR amplification reactions performed with EasyTaq® DNA Polymerase. Features • Standalone buffer. • … shire of ravensthorpe map