WebCitrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern. [ 1] アプリケーション WebUnit Definition: One unit of Citrate Synthase is the amount of enzyme that will generate 1.0 µmol CoA per min. at pH 7.2 at 25ºC. Figure: (a) GSH Standard Curve. (b) Citrate Synthase Activity in Jurkat cell lysate (10 µg), rat liver lysate (20 µg), purified yeast mitochondria (4 µg) and CS Positive Control (2 µl).
Biomarkers of mitochondrial content in skeletal muscle of ... - PubMed
WebCitrate synthase (CS) catalyzes the first reaction of the TCA cycle and is generally assumed to be the rate-limiting enzyme of the cycle [3]. Increasing evidence suggests that CS activity is closely associated with various kinds of cancers. The activity of citrate synthase was measured using tissue extract prepared from WebJun 1, 2005 · Maximal activity of citrate synthase (CS) measured in resting muscle biopsy samples obtained before and after a 2-wk sprint training protocol. Values are means ± SE for 8 subjects, ww, Wet wt. * P < 0.05. Download figure Download PowerPoint Fig. 5. colouring in sheep
K318-100 Citrate Synthase Activity Assay Kit - BioVision
WebApr 7, 2024 · Citrate synthase content measurement. Citrate synthase (CS) activity is a commonly used marker of mitochondrial volume. 38 Maximal CS activity in RBCs was assayed at 41°C following Dawson et al. 11 Briefly, thawed samples were kept on ice and homogenized in 2.5 volumes of homogenizing buffer [100 mmol L −1 KH 2 PO 4 buffer, … WebCitrate synthase (EC 2.3.3.1, formerly 4.1.3.7; (S -)citrate synthase; CS) is the first of a series of eight enzymes involved in the citric acid cycle. The citric acid cycle is also … WebTotal Citrate Synthase Activity – For the detection of total citrate synthase activity in mitochondria, suspend the prepared mitochondrial pellet in CelLytic M Cell Lysis Reagent (Catalog Number C2978) using ∼200 µl per g of tissue or per 2–5 × 107 cells. Inner Mitochondrial Membrane Intactness Assay – dr taylor georgetown ky